Jolanta Bryjak

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Department of Bioorganic Chemistry

Wrocław University of Technology

ul. Norwida 4/6

50-373 Wrocław

phone: (+48 71) 320 26 77


Hobby: gardening, travelling

Status: married, two daughters


Immobilization and stabilisation of enzyme activity - adsorption, crosslinking, copolymerization, covalent attachment, stabilization by additives. Membrane processes- ultra- and microfiltration, immobilisation of enzymes on/in membranes, membrane selection for bioprocesses. Catalytic characterization and kinetic behaviour of enzymes in native and immobilized forms. Bioreactors - batch and continuous reactors, reactors in series, membrane reactors, fluidized and packed bed reactors. Enzyme purification by: flocculation, affinity chromatography, hydrophobic interaction chromatography, membrane fractionation Mathematical modelling of enzymatic processes and enzyme inactivation by phenomenological models and artificial neural network.


1980 - M.Sc., Institute of Plant Physiology, Department of Biology, Warsaw University

1990 - Ph.D., Institute of Chemical Engineering, Department of Chemistry, Technical University of Wrocław

2005 - D.Sc., Department of Food Technology, Wrocław University of Environmental and Life Sciences


University of Calabria, Arcavata di Rende, 1994 University of Bath, Bath, 1995 Stuttgart University, Stuttgart, 1996 Slovak Technical University, Bratislava, permanent collaboration since1996

Last Publications

  1. Tataruch, M.; Heider, J.; Bryjak, J.; Nowak, P.; Knack, D.; Czerniak A.; Lesiene, J.; Szaleniec M., Suitability of the hydrocarbon-hydroxylating molybdenum-enzymeethylbenzene dehydrogenase for industrial chiral alcohol production J. Biotechnol. 2014, 192, 400. View at Publisher
  2. Hrydziuszko, Z.; Dmytryk, A.; Majewska, P.; Szymańska, K.; Liesiene, J.; Jarzębski, A.; Bryjak, J., Screening of lipase carriers for reactions in water, biphasic and pure organic solvent systems Acta Biochim. Polon. 2014, 61, 1. View at Publisher
  3. Feder-Kubis, J.M.; Bryjak, J., Laccase activity and stability in the presence of menthol-based ionic liquids. Acta Biochim. Polon. 2013, 60, 741. View at Publisher
  4. Labus, K.; Gancarz, I. and Bryjak, J., Immobilization of laccase and tyrosinase on untreated and plasma-treated cellulosic and polyamide membranes Mat. Sci. Eng. C 2012, 32, 228. View at Publisher
  5. Bryjak, J.; Szymańska, K.; Jarzębski, A., Laccase immobilisation on mesostructured silicas. Chem. Proc. Eng. 2012, 33, 611. View at Publisher
  6. Zynek, K.; Bryjak, J.; Szymanska, K. and Jarzebski, A .B., Screening of Porous and Cellular Materials for Covalent Immobilisation of Agaricus bisporus Tyrosinase Biotechnol. Bioproc. Eng. 2011, 16, 180. View at Publisher
  7. Labus, K.; Turek, A.; Liesiene, J. and Bryjak, J., Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers Biochem. Eng. J. 2011, 56, 232. View at Publisher
  8. Jakób, A.; Bryjak, J.; Wójtowicz, H.; Illeová, V.; Annus, J.; Polakovič, M., Inactivation kinetics of food enzymes during ohmic heating. Food Chem. 2010, 123, 369.
  9. Rekuć, A.; Bryjak, J.; Szymańska, K.; Jarzębski, A.B., Very stable silica-gel-bound laccase biocatalysts for the selective oxidation in continuous systems. Biores.Tech. 2010, 101, 2076.
  10. Onderková, Z.; Bryjak, J.; Vaňková, K.; Polakovič, M., Kinetics of thermal inactivation of free Aureobasidium pullulans fructosyltransferase. Enzyme Microb. Tech. 2010, 47, 134.
  11. Bryjak, J.; Rekuć, A., Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation. App. Biochem. Biotech. 2010, 160, 2219.
  12. Labus, K.; Bryjak, J.; Polakovič, M., Effect of separation on thermal stability of tyrosinase from Agaricus bisporus. J. Mol. Catal. B Enzym. 2010, 66, 172.
  13. Jaworska, M.M.; Bryjak, J.; Liesiene, J., A search of an optimal carrier for immobilization of chitin deacetylase. Cellulose 2009, 16, 261.
  14. Cabaj, J.; Sołoducho, J.; Chyla, A.; Bryjak, J.; Zynek, K., The characterization of ordered thin films built of immobilized phenoloxidases. Sensors Actuators B 2009, 136, 425.
  15. Połata, H.; Wilińska, A.; Bryjak, J.; Polakovic, M., Thermal inactivation kinetics of vegetable peroxidases. J. Food Eng. 2009, 91, 387.
  16. Rekuć, A.; Jastrzembska, B.; Liesiene, J.; Bryjak, J., Comparative studies on immobilized laccase behaviour in packed-bed and batch reactors. J. Mol. Catal. B: Enzym. 2009, 57, 216.
  17. Rekuć, A.; Bryjak, J.; Szymańska, K.; Jarzębski, A.B., Laccase immobilization on mesostructured cellular foams affods preparations with ultra high activity. Proc. Biochem. 2009, 44, 191.
  18. Gancarz, I.; Bryjak, J.; Labus, K., Chemical modification of poly(ethylene terephthalate) and immobilization of the selected enzymes on the modified film. App. Surf. Sci. 2009, 255, 8293.
  19. Szymańska, K.; Bryjak, J.; Jarzębski, A.B., Immobilization of invertase on mesoporous silicas to obtain hyper active biocatalysts. Top. Catal. 2009, 52, 1030.
  20. Jakób, A.; Bryjak, J.; Polakovič, M., Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials. Chem. Pap. 2009, 63, 677.
  21. Wilińska, A.; Figueiredo Rodrigues, A.S.; Bryjak, J.; Polakovic, M., Thermal inactivation of exogenous pectin methylesterase in apple and cloudberry juice. J. Food Eng. 2008, 85, 459.
  22. Rekuć, A.; Kruczkiewicz, P.; Jastrzembska, B.; Liesiene, J.; Peczyńska-Czoch, W.; Bryjak, J., Laccase immobilization on the tailored cellulose-based Granocel carriers. Int. J. Biol. Macromol. 2008, 42, 208.
  23. Cabaj, J.; Idzik, K.; Sołoducho, J.; Chyla, A.; Bryjak, J.; Doskocz, J., Well-ordered thin films as practical components of biosensors. Thin Layer Films 2008, 516, 1171.
  24. Bryjak, J.; Liesiene, J.; Kolarz, B., Application and properties of butylacrylate/pentaerythrite triacrylate copolymers and cellulose-based Granocel as carriers for trypsin immobilization. Coll. Surf. B: Biointerfaces 2008, 61, 66.
  25. Bryjak, J.; Liesiene, J.; Stefuca, V., Man-tailored cellulose-based carriers for invertase immobilization. Cellulose 2008, 15, 631.
  26. Onderkova, Z.; Polakovic, M., Properties of fructosyltransferase from Aureobasidium pullulans immobilized on an acrylic carrier. Chem. Papers 2007, 61, 359.
  27. Hamerska-Dudra, A.; Bryjak, J.; Trochimczuk, A., Immobilization of glucoamylase and trypsin on crosslinked thermosensitive carriers. Enzyme Microb. Technol. 2007, 41, 197.
  28. Jarzębski, A.B.; Szymańska, K.; Bryjak, J.; Mrowiec-Białoń, J., Covalent immobilization of trypsin on to siliceous mesostructured cellular foams to obtain effective biocatalysts. Catal. Today 2007, 124, 2.
  29. Bryjak, J.; Kruczkiewicz, P.; Rekuć, A.; Peczyńska-Czoch, W., Laccase immobilization on copolymer of butyl acrylate and ethylene glycol dimethacrylate. Biochem. Eng. J. 2007, 35, 325.
  30. Bryjak, J.; Aniulyte, J.; Liesiene, J., Evaluation of man-tailored cellulose-based carriers in glucoamylase immobilization. Carbohydr. Res. 2007, 342, 1105.
  31. Szymańska, K.; Bryjak, J.; Mrowiec-Białoń, J.; Jarzębski, A.B., Application and properties of siliceous mesostructured cellular foams as enzymes carriers to obtain efficient biocatalysts. Micropor. Mesopor. Mater. 2007, 99, 167.
  32. Wilińska, A.; Bryjak, J.; Illeova, V.; Polakovic, M., Kinetics of thermal inactivation of alkaline phosphatase in bovine and caprine milk and buffer. Int. Diary J. 2007, 17, 579.
  33. Gancarz, I.; Bryjak, J.; Bryjak, M.; Tylus, W.; Pozniak, G., Poly(phenylene oxide) films modified with allylamine plasma as a support for invertase immobilization. Eur. Polymer J. 2006, 42, 2430.
  34. Bryjak, J.; Trochimczuk, A.W., Immobilization of lipase and penicillin acylase on hydrophobic acrylic carriers. Enzyme Microb. Tech. 2006, 39, 573.
  35. Hamerska-Dudra, A.; Bryjak, J.; Trochimczuk, A.W., Novel method of enzymes stabilization on crosslinked thermosensitive carriers. Enzyme Microb. Tech. 2006, 38, 921.
  36. Aniulyte, J.; Bryjak, J.; Liesiene, J., Activation of cellulose-based carriers with pentaethylenehaxamine. Proc. Estonian Acad. Sci. Chem. 2006, 55, 61.