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Department of Bioorganic Chemistry is one of 15 departments of Faculty of Chemistry, Wrocław University of Technology. It was founded in 1971 by Professor Przemysław Mastalerz in response to introduction of biochemistry and biotechnology curricula at Faculty of Chemistry. Now it is headed by Professor Paweł Kafarski and formed by 6 professors, 10 assistant professors and over 25 PhD students doing research on a border of chemistry and biology.


Members

Research

Publications


Symposium on Biotransformations for Pharmaceutical and Cosmetic Industry



Recent papers

Fungal synthesis of chiral phosphonic synthetic platform – Scope and limitations of the method
Serafin-Lewańczuk M, Klimek-Ochab M,Brzezińska-Rodak M, Żymańczyk-Duda E
Bioorganic Chemistry 2018, 77, 402
DOI: 10.1016/j.bioorg.2018.01.027

Recent papers

Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1
Gonzalez-Ramirez ML*, Poreba M*, Snipas SJ, Groborz K, Drag M, Salvesen GS (* equal contribution)
Journal of Biological Chemistry 2018, 1
DOI: 10.1074/jbc.RA117.001329

Recent papers

Selective imaging of cathepsin L in breast cancer by fluorescent activity-based probes
Poreba M, Rut W, Vizovisek M, Groborz K, Kasperkiewicz P, Finlay D, Vuori K, Turk D, Turk B, Salvesen GS, Drag M
Chemical Science 2018, 1
DOI: 10.1039/c7sc04303a

Recent papers

Nanokrzemionka - bioperspektywa i potencjał przemysłowy. Cz. 2.
Fajfer, D.; Zielonka, A.; Klimek-Ochab, M.
Laboratorium-Przegląd Ogólnopolski 2017, 32

Recent papers

Chemical Profiling of Polyfloral Belgian Honey: Ellagic Acid and Pinocembrin as Antioxidants and Chemical Markers
Jasicka-Misiak, I.; Gruyaert, S.; Poliwoda, A., Kafarski, P.
J. Chem. 2017, 2017, 1
DOI: 10.1155/2017/5393158

Abstract
Chemical profiling of northern Belgian polyfloral honeys was performed to analyse their phenolic compound content (flavonoids and phenolic acids). First, samples were subjected to a standard analysis of their physicochemical properties, and then, the phenolic fraction was isolated and analysed using a HPLC/PAD method. All of the tested honeys showed a common and specific phenolic profile that could be the basis for the differentiation of polyfloral honeys of the Antwerp region from other polyfloral honeys. Chromatographic data indicated a high content of ellagic acid (9.13–13.66 mg/100 g honey), as well as the flavonoid pinocembrin (1.60–1.85 mg/100 g honey) in these honeys. Ellagic acid, a compound with well-defined prohealth activities, might be used as a chemical marker for these honeys. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys.

Recent papers

Whole-cell biotransformation of diethyl 1-hydroxy-1-phenylmethanephosphonate in a different reaction environment.
P. Majewska
Phosphorus, Sulfur, and Silicon and the Related Elements 2017, 192, 418

Recent papers

Yeast as a Versatile Tool in Biotechnology
Zymańczyk-Duda E, Brzezińska-Rodak M, Klimek-Ochab M, Duda M, Zerka A
Yeast - Industrial Applications, Prof A.Morata (Ed) InTech 2017, 1
DOI: 10.5772/intechopen.70130

Recent papers

Nanokrzemionka - bioperspektywa i potencjał przemysłowy. Cz. 1.
Fajfer D., Zielonka A., Klimek-Ochab M.
Laboratorium-Przegląd Ogólnopolski 2017, 49
Abstract
Nanotechnologia jest bardzo szybko rozwijającą się dziedziną nauki. W ostatnich latach szczególną uwagę poświęcono cząstkom o wymiarach nano, które posiadają szeroki zakres zastosowań w przemyśle spożywczym, papierniczym, ceramicznym, budowlanym, medycznym oraz rolnym. Jedną z takich nanocząstek jest nanokrzemionka, której synteza biometodami zyskuje coraz większe zainteresowanie ze względu na ich ekonomiczność oraz neutralność wobec środowiska. W niniejszej publikacji przedstawiono formę występowania nanokrzemionki wykorzystywaną podczas biokonwersji roślin, które są bogate w ten cenny składnik. Zaprezentowano również mechanizm jej biopozyskiwania w porównaniu z metodami chemicznymi i fizycznymi, a także jej szeroki zakres aplikacyjny.

Recent papers

Fungal synthesis of size-defined nanoparticles
Zielonka A., Klimek-Ochab M.
Advances in Natural Sciences-Nanoscience and Nanotechnology 2017, 8, 1
DOI: https://doi.org/10.1088/2043-6254/aa84d4
Abstract
Fungi with metabolic capacities can efficiently synthesize a wide range of nanoparticles (NPs).

This biotransformation process and its product have extensive applications especially for industry, agriculture and medicine, where NPs size and shape is essential and can be defined by specific analytical methods. Fungi cultivation and further bioconversion can be fully controlled to obtain the desired nanoparticles. Additionally, this review provides information about the

fungus F. oxysporum, which is able to synthesize the largest amount of different types of NPs.

Recent papers

New aspects of coordination chemistry and biological activity of NTMP-related diphosphonates containing a heterocyclic ring
Gałęzowska, J.; Czapor-Irzabek, H.; Janicki, R.; Chmielewska, E.; Janek, T.
New J. Chem. 2017, 41, 10731
DOI: 10.1039/c7nj02415k

Abstract
Two analogues of nitrilotris(methylene-phosphonic acid) (NTMP), namely L1 = N-(methylene-2-pyridine)-N,N,-di-(methylenephosphonate) and L2 = N-(methylene-1H-benzimidazol)-N,N,-di-(methylenephosphonate)in which one of the phosphonic arms was replaced by a heterocyclic moiety, pyridine and benzimidazole, were studied in terms of coordination chemistry towards transition (Cu2+, Ni2+, Zn2+) and alkaline-earth metal ions (Ca2+ and Mg2+) by means of potentiometry, UV-vis spectroscopy, mass spectrometry (ESI-MS), and isothermal titration calorimetry (ITC). The cytotoxicity of the ligands as well as their Zn2+, Ca2+ and Mg2+ complexes was tested against various cell lines (human melanoma A375 and human colon adenocarcinoma HT29) revealing a selective antitumor effect in vivo. Both of the ligands exhibit a potent inhibitory effect on tumor cell migration and experimental metastasis. Potentiometric and ESI-MS measurements have shown the existence of monomeric species only, without the presence of biscomplexes or polynuclear species. The conditional stability constants (log Kc) of the Zn2+, Ca2+, and Mg2+ complexes were determined independently by two methods for both of the studied ligands and are a first example of comprehensive potentiometry/ITC studies made for phosphonic acid complexation. No significant differentiation in coordination models for the studied set of metal ions was noticed; however, as expected due to the different metal ion natures, the complexes revealed dissimilar thermodynamic stability and behavior, depending on the metal ion and pH used. The possible structures of the complexes formed are discussed on the basis of spectroscopic and spectrometric results.