Marcin Poręba

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Poreba.jpg

Married with Aleksandra, son Tobiasz (2016)

Born: Lubin, Poland, April 25, 1986

Hobby: football, action films, music (Rysiek Riedel, Maleńczuk)

e-mail: marcin.poreba@pwr.edu.pl

Phone: (+48) 71 320 2455


Education

M.Sc., 2010 Wroclaw University of Technology, Poland, “Substrate mapping of the malaria leucyl aminopeptidase using substrates library” supervisor: Marcin Drag, PhD

Ph.D., 2014 Wroclaw University of Technology, Poland, “Design and synthesis of substrate libraries containing natural and unnatural amino acids for human cysteine proteases - caspases and cathepsins” supervisor: Marcin Drag, PhD, DSc

Awards and honours

2015 October Travel Award - The 9th General Meeting of the International Proteolysis Society, 04-08.10.2015, Penang, Malaysia

2015 April Marie Curie Global Fellowship, project title: "Protease visualization during tumor progression - PROVIST"

2015 March Award for one of the best talk at "XXXII Winter School on Proteinases and Their Inhibitors", Tiers, Italy

2014 April START scholarship founded by the Fundation for Polish Science

2013 December Scholarship of the Ministry of Science and Higher Education for scientific achievements

2013 October Award of President of Wroclaw University of Technology for outstanding scientific achievements

2013 October Beneficiary of GRANT PLUS programme founded by Human Capital Operational Programme European Union

2013 October Travel Award - The 8th General Meeting of the International Proteolysis Society, 20-24.10.2013, CapeTown, RSA

2013 September Beneficiary of the Project "Development of the Teaching & Research Capacity of Young Academic Staff at Wrocław University of Technology"

2013 April START scholarship founded by the Fundation for Polish Science

2012 October Award of President of Wroclaw University of Technology for outstanding scientific achievements

2012 April Invited speaker at the “Gordon Research Seminar on Proteolytic Enzymes and their Inhibitors” Il Ciocco, Italy 16-17.06.2012

2012 February Award of President of Wroclaw University of Technology for distinctive contribution in organization and participation in XIII Lower Silesia Festival of Science

2011 October Award of President of Wroclaw University of Technology for outstanding scientific achievements

2011 September Beneficiary of Enterprising graduate student – an investment in innovative development of the region. Human Capital Operational Programme European Union (Przedsiębiorczy doktorant - Program Operacyjny Kapitał Ludzki) (1.10.2011-31.03.2013)

2011 September Scholarship of President of Wroclaw University of Technology for scientific achievements (for the 2nd year of Ph.D. study)

2011 April Scholarship from the programme: „Rozwój potencjału dydaktyczno-naukowego Młodej Kadry Akademickiej Politechniki Wrocławskiej” (4th edition 1.04.2011-31.09.2011)

2011 April Award of President of Wroclaw University of Technology for outstanding scientific achievements

2010 September Scholarship from the programme: „Rozwój potencjału dydaktyczno-naukowego Młodej Kadry Akademickiej Politechniki Wrocławskiej” (3rd edition 1.10.2010-31.03.2011)

2010 September Scholarship of President of Wroclaw University of Technology for scientific achievements (for the 1st year of Ph.D. study)

2010 September Award “Editorial`s choice” for review paper „Current strategies for probing substrate specificity of proteases” published in Current Medicinal Chemistry

Publications (from ISI Master Journal List)

  1. Balogh B, Dahmen M, Stahl M, Poreba M, Gersch M, Drag M, Sieber SA , Insights into ClpXP proteolysis: heterooligomerization and partial deactivation enhance chaperone affinity and substrate turnover in Listeria monocytogenes Chemical Science 2017, 8, 1592. View at Publisher
  2. Rut W, Zhang L, Kasperkiewicz P, Poreba M, Hilgenfeld R, Drag M, Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 protease Antiviral Research 2017, 139, 88. View at Publisher
  3. Kasperkiewicz P, Poreba M, Groborz K, Drag M, Emerging challenges in the design of selective protease substrates, inhibitors and activity-based probes for indistinguishable proteases FEBS Journal 2017, 284, 1518. View at Publisher
  4. Drag-Zalesinska M, Drag M, Poreba M, Borska S, Kulbacka J, Saczko J, Anticancer properties of ester derivatives of betulin in human metastatic melanoma cells (Me-45) Cancer Cell International 2017, 17, 7. View at Publisher
  5. Poreba M, Szalek A, Rut W, Kasperkiewicz P, Rutkowska-Wlodarczyk I, Snipas SJ, Itoh Y, Turk D, Turk B, Overall CM, Kaczmarek L, Salvesen GS, Drag M, Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families Scientific Reports 2017, 7, 43135. View at Publisher
  6. Poreba M, Salvesen GS, Return of the Ice Age: Caspases Safeguard against Inflammatory Cell Death Cell Chemical Biology 2017, 24, 550. View at Publisher
  7. Lunde NN, Haugen MH, Bodin Larsen KB, Damgaard I, Pettersen SJ, Kasem R, Rut W, Drag M, Poreba M, Johansen HT, Solberg R, Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions Biochimie 2017, 139, 27. View at Publisher
  8. Poreba M, Salvesen GS, Drag M, Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity Nature Protocols 2017, 12, 2189. View at Publisher
  9. Gersch M, Stahl M, Poreba M, Dahmen M, Dziedzic A, Drag M, Sieber SA, Barrel-shaped ClpP proteases display attenuated cleavage specificity ACS Chemical Biology 2016, 11, 389. View at Publisher
  10. Poreba M, Solberg R, Rut W, Lunde NN, Kasperkiewicz P, Snipas SJ, Mihelic M, Turk D, Turk B, Salvesen GS, Drag M., Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes Cell Chemical Biology 2016, 23, 1023. View at Publisher
  11. Rut, W.; Kasperkiewicz, P.; Byzia, A.; Poreba, M.; Groborz, K.; Drag, M., Recent advances and concepts in substrate specificity determination of proteases using tailored libraries of fluorogenic substrates with unnatural amino acids Biol. Chem. 2015, 396, 329. View at Publisher
  12. Hachmann J, Edgington-Mitchell LE, Poreba M, Sanman LE, Drag M, Bogyo M, Salvesen GS., Probes to monitor activity of the paracaspase MALT1 Chemistry & biology 2015, 22, 139. View at Publisher
  13. Drag-Zalesinska M, Wysocka T, Borska S, Drag M, Poreba M, Choromanska A, Kulbacka J, Saczko J., The new esters derivatives of betulin and betulinic acid in epidermoid squamous carcinoma treatment - In vitro studies. Biomedicine & Pharmacotherapy 2015, 72, 91. View at Publisher
  14. Kasperkiewicz P, Poreba M, Snipas SJ, Lin SJ, Kirchhofer D, Salvesen GS, Drag M., Design of a Selective Substrate and Activity Based Probe for Human Neutrophil Serine Protease 4. PLoS One 2015, 10, 1. View at Publisher
  15. Rajkovic, J.; Poreba, M.; Caglic, D.; Vidmar, R.; Wilk, A.; Borowik, A.; Salvesen, G. S.; Turk, V.; Drag, M.; Turk, B., Biochemical Characterization and Substrate Specificity of Autophagin-2 from the Parasite Trypanosoma cruzi J. Biol. Chem. 2015, 290, 28231. View at Publisher
  16. Galiullina RA, Kasperkiewicz P, Chichkova NV, Szalek A, Serebryakova MV, Poreba M, Drag M, Vartapetian AB, Substrate Specificity and Possible Heterologous Targets of Phytaspase, a Plant Cell Death Protease. Journal of Biological Chemistry 2015, 290, 24806. View at Publisher
  17. Poreba M, Szalek A, Kasperkiewicz P, Rut W, Salvesen GS, Drag M, Small Molecule Active Site Directed Tools for Studying Human Caspases Chemical Reviews 2015, 115, 12546. View at Publisher
  18. Poręba, M.; Mihelic, M.; Krai, P.; Rajkovic, J.; Krężel, A.; Pawełczak, M.; Klemba, M.; Turk, D.; Turk, B.; Latajka, R.; Drąg, M., Unnatural amino acids increase activity and specificity of synthetic substrates for human and malarial cathepsin C. Amino Acids 2014, 46, 931. View at Publisher
  19. Kasperkiewicz, P.; Poreba, M.; Snipas, S. J.; Parker, H.; Winterbourn, C. C.; Salvesen, G. S.; Drag, M., Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling. Proc. Natl. Acad. Sci. U. S. A. 2014, 111, 2518. View at Publisher
  20. Poręba, M.; Szalek, A.; Kasperkiewicz, P.; Drąg, M., Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity. Methods Mol. Biol. 2014, 1133, 41. View at Publisher
  21. Poreba, M.; Kasperkiewicz, P.; Snipas, S. J.; Fasci, D.; Salvesen, G. S.; Drąg, M., Unnatural amino acids increase sensitivity and provide for the design of highly selective caspase substrates Cell Death & Differentiation 2014, 21, 1482. View at Publisher
  22. Węglarz-Tomczak, E.; Poręba, M.; Byzia, A.; Berlicki, Ł.; Nocek, B.; Mulligan, R.; Joachimiak, A.; Drąg, M.; Mucha, A., An integrated approach to the ligand binding specificity of Neisseria meningitidis M1 alanine aminopeptidase by fluorogenic substrate profiling, inhibitory studies and molecular modeling. Biochimie 2013, 95, 419. View at Publisher
  23. Carroll, R.K.; Veillard, F.; Gagne, D.T.; Lindenmuth, J.M.; Poreba, M.; Drag, M.; Potempa, J.; Shaw, L.N., The Staphylococcus aureus leucine aminopeptidase LAP is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine. Biol. Chem. 2013, 394, 791. View at Publisher
  24. Zeiler, E.; List, A.; Alte, F.; Gersch, M.; Wachtel, R.; Poręba, M.; Drąg, M.; Groll, M.; Sieber, S.A., Structural and functional insights into caseinolytic proteases reveal an unprecedented regulation principle of their catalytic triad. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 11302. View at Publisher
  25. Poręba, M.; Stróżyk, A.; Salvesen, G.S.; Drag, M. , Caspase Substrates and Inhibitors. Cold Spring Harb. Perspect. Biol. 2013, 5, 1. View at Publisher
  26. Poreba, M.; McGowan, S.; Skinner-Adams, T. S.; Trenholme, K. R.; Gardiner, D. L.; Whisstock, J. C.; To, J.; Salvesen, G. S.; Dalton, J. P.; Drąg, M., Fingerprinting the Substrate Specificity of M1 and M17 Aminopeptidases of Human Malaria, Plasmodium Falciparum. PLoS One 2012, 7, 31938. View at Publisher
  27. Hachman, J.; Snipas, S. J.; Van Raam, B. J.; Cancino, E. M.; Houlihan, E. J.; Poręba, M.; Kasperkiewicz, P.; Drąg, M.; Salvesen, G. S., Mechanism and specificity of the human paracaspase MALT1. Biochem. J. 2012, 443, 287. View at Publisher
  28. Poręba, M.; Gajda, A.; Picha, J.; Jiracek, J.; Marschner, A.; Klein, C.; Salvesen, G. S.; Drąg, M., S1 pocket fingerprints of human and bacterial methionine aminopeptidases determined using fluorogenic libraries of substrates and phosphorus based inhibitors. Biochimie 2012, 94, 704. View at Publisher
  29. Sivaraman, K.K.; Oellig, C.A.; Huynh, K.; Atkinson, S.C.; Poreba, M.; Perugini, M.A.; Trenholme, K.R.; Gardiner, D.L.; Salvesen, G.; Drag, M.; Dalton, J.P.; Whisstock, J.C.; McGowan, S., X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase PfM18AAP. J. Mol. Biol. 2012, 422, 495. View at Publisher
  30. Veillard, F.; Potempa, B.; Poreba, M.; Drag, M.; Potempa, J., Gingipain aminopeptidase activities in Porphyromonas gingivalis. Biol. Chem. 2012, 393, 1471. View at Publisher
  31. Zervoudi, E.; Papakyriakou, A.; Georgiadou, D.; Evnouchidou, I.; Gajda, A.; Poręba, M.; Salvesen, G. S.; Drąg, M.; Hattori, A.; Swevers, L.; Vourloumis, D.; Stratikos, E., Probing the S1 specificity pocket of the aminopeptidases that generate antigenic peptides. Biochem J. 2011, 435, 411. View at Publisher
  32. Poręba, M.; Drąg, M., Current strategies for probing substrate specificity of proteases. Curr. Med. Chem. 2010, 17, 3968. View at Publisher

Publications (in polish)

  1. Poreba, M.; Kaspazy inicjatorowe i egzekucyjne w kontekście projektowania małocząsteczkowych markerów chemicznych Nowe trendy w naukach przyrodniczych – monografie. 2012, tom V, 141.
  2. Barwinek, D.; Poreba, M.; Drag. M; Substraty fluorogeniczne zawierające nienaturalne aminokwasy jako narzędzie w badaniu kaspaz. Prace Badawcze Studentów Politechniki Wrocławskiej, 2012, 10, 13.
  3. Poreba, M.; Kasperkiewicz, P., Narzędzia chemii biologicznej w badaniu proteaz. Nowe trendy w naukach przyrodniczych – monografie. 2012, tom II, 112.
  4. Kasperkiewicz, P.; Poreba, M., Ludzka neutrofilowa elastaza (HNE) – przegląd. Nowe trendy w naukach przyrodniczych – monografie. 2012, tom II, 187.


Conference communications

  1. Poreba M, Rut W, Rutkowska I, Kaczmarek L, Overall Ch, Drag M; New selective internally quenched substrates for the investigation of human matrix mettaloproteinases 2 and 9; The 9th General Meeting of the International Proteolysis Society, 04-08.10.2015, Penang, Malaysia (poster)
  2. Poreba M, Kasperkiewicz P, Turk B, Drag M; HyCoSuL approach fot the optimization of cathepsin L substrate specificty; XXXII Winter School on Proteinases and Their Inhibitors, 25.02.-01.03.2015, Tiers am Rosegarden, Italy (oral presentation).
  3. Poreba M, Kasperkiewicz P, Rut W, Snipas SJ, Salvesen GS, Drag M; Unnatural amino acids as a new tool for investigation of substrate specificity of human caspases; VI Conversatory on Medicinal Chemistry, 18.09.-20.09.2014, Lublin, Poland (oral presentation).
  4. Poreba M, Kasperkiewicz P, Rut W, Snipas SJ, Salvesen GS, Drag M; HyCoSuL approach as a tool for identification of new, specific substrates and inhibitors for human caspase 2; XIVth Symposium on Proteases, Inhibitors and Biological Control, 06.09.-10.09.2014, Portoroz, Slovenia (poster).
  5. Poreba M, Kasperkiewicz P, Rut W, Snipas SJ, Salvesen GS, Drag M; HyCoSuL as a new tool providing for the design of caspase specific substrates; Gordon Research Conference on Proteolytic Enzymes and their Inhibitors, 22.07.-27.07.2014, Il Ciocco, Italy (poster).
  6. Poreba M, Kasperkiewicz P, Rut W, Mihelic M, Snipas SJ, Turk B, Turk D, Salvesen GS, Drag M; Design of highly specific substrates and activity probes for legumain through HyCoSuL profiling; Gordon Research Seminar on Proteolytic Enzymes and their Inhibitors, 21.07.-22.07.2014, Il Ciocco, Italy (poster).
  7. Poreba M, Kasperkiewicz P, Rut W, Snipas SJ, Turk D, Salvesen GS, Drag M; Unnatural amino acids provide for the design of highly selective substrates for caspases and legumain; Pacific Coast Protease Spring School, 29.04.-02.05.2014, Miracle Springs Resort & Spa Desert Hot Springs, California, USA (oral presentation).
  8. Poreba M, Kasperkiewicz P, Rut W, Mihelic M, Turk D, Turk B, Drag M; Legumain substrate specificity determined by Hybrid Combinatorial Substrate Library (HyCoSuL) approach; XXXIst Winter School on Proteinases and Their Inhibitors, 26.02.-02.03.2014, Tiers am Rosengarten, Italy (oral presentation).
  9. Poreba M, Kasperkiewicz P, Rut W, Snipas SJ, Mihelic M, Turk B, Salvesen GS, Drag M.; The use of unnatural amino acids in peptide fluorogenic substrates reveals unexpected active site preferences of human caspases and legumain; The 8th General Meeting of the International Proteolysis Society, 20-24.10.2013, CapeTown, RSA (poster).
  10. Poreba M, Kasperkiewicz P, Snipas SJ, Salvesen GS, Drag M.; Initiator caspases can be distinguished by small molecule fluorogenic substrates containing unnatural amino acids. XXXth Winter School on Proteinases and Their Inhibitors, 27.02-03.03.2013, Tiers, Italy (oral presentation).
  11. Poreba M, Kasperkiewicz P, Drag M.; Hybrid combinatorial library of flurogenic substrates as a new tool for profiling active site specificity of human caspases; XIIIth Symposium on Proteases, Inhibitors and Biological Control, 22-26.09.2012, Portoroz, Slovenia (poster).
  12. Poreba M, Kasperkiewicz, Drag M.; Fluorogenic substrate libraries with unnatural amino acids as tools for investigation of important human cysteine proteases; Gordon Research Conference on Proteolytic Enzymes and their Inhibitors; 17-22.06.2012, Il Ciocco, Italy (poster).
  13. Poreba M, Kasperkiewicz, Drag M.; Fluorogenic substrate libraries with unnatural amino acids as tools for investigation of important human cysteine proteases; Gordon Research Seminar on Proteolytic Enzymes and their Inhibitors; 16-17.06.2012, Il Ciocco, Italy (oral presentation).
  14. Poreba M, Kasperkiewicz P, Snipas SJ, Salvesen GS, Drag M.; Combinatorial chemistry-based approach for profiling human caspase 3; Na pograniczu biologii i chemii; 05.2012, Ustron, Poland (oral presentation).
  15. Poreba M, Kasperkiewicz, P., Gajda, A., Drąg, M.; Combinatorial substrate library as a tool for investigation of important human proteases. Studies on substrate specificity of cathepsin C; 21st Polish Peptide Symposium, 4-8.09.2011, Suprasl, Poland (oral presentation).
  16. Poreba M, Kasperkiewicz, P., Gajda, A., Drag, M.; Protease based drug discovery. Studies on substrate specificity of cathepsin C Young Scientists Forum, 30.06-1.07.2011, Warsaw, Poland (poster).
  17. Poreba M, Gajda, A., Pawelczak, M., Marschner, A., Klein, Ch., Bogyo, M., Ellman, J.A., Salvesen, G.S., Drag, M.; Substrate Specificity of medicinally important human aminopeptidases; 12th International Symposium on proteases, inhibitors and biological control; 25-29.09.2010; Portoroz, Slovenia (poster).
  18. Poreba M, Jewginski, M., Pawełczak, M., Latajka, R., Drąg, M., Substrate specificity of cathepsin C (DPPI) determined using broad range of natural and unnatural amino acids; 12th International Symposium on proteases, inhibitors and biological control; 25-29.09.2010; Portoroz, Slovenia (poster).