Difference between revisions of "Nanostructures based on peptide foldamers"

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'''Scientific Cooperation''':
 
'''Scientific Cooperation''':
Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology: Małgorzata Kotulska, PhD, D.Sc, Eng; Marlena Gąsior-Głogowska, PhD, Eng; Natalia Szulc, MSc, Eng. FTIR, RAMAN
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Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology:  
 +
 
 +
Małgorzata Kotulska, PhD, D.Sc, Eng; Marlena Gąsior-Głogowska, PhD, Eng; Natalia Szulc, MSc, Eng.  
 +
 
 +
FTIR, RAMAN
  
 
Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology:
 
Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology:
 +
 
Teodor Gotszalk, PhD, D.Sc, Eng; Krzysztof Gajewski, PhD, Eng; Wojciech Majstrzyk, MSc, Eng.
 
Teodor Gotszalk, PhD, D.Sc, Eng; Krzysztof Gajewski, PhD, Eng; Wojciech Majstrzyk, MSc, Eng.
 
AFM
 
AFM
  
 
Faculty of Mechanical Engeineering, Wroclaw University of Science and Technology:
 
Faculty of Mechanical Engeineering, Wroclaw University of Science and Technology:
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Andrzej Żak, PhD, Eng;
 
Andrzej Żak, PhD, Eng;
 +
 
TEM
 
TEM
  
 
Division of Biophysics, Faculty of Physics, Institute of Experimental Physics, University of Warsaw,  
 
Division of Biophysics, Faculty of Physics, Institute of Experimental Physics, University of Warsaw,  
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Agnieszka Bzowska, , PhD, D.Sc; Anna Modrak-Wójcik, PhD
 
Agnieszka Bzowska, , PhD, D.Sc; Anna Modrak-Wójcik, PhD
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AUC
 
AUC
  
 
Faculty of Biochemistry, Molecular Biology and Biotechnology, Wroclaw University of Science and Technology:
 
Faculty of Biochemistry, Molecular Biology and Biotechnology, Wroclaw University of Science and Technology:
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Rafał Hołubowicz, PhD, Eng;
 
Rafał Hołubowicz, PhD, Eng;
AUC
 
  
  
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The main goal of the project involves rational design of peptide foldamers to be exploited as building blocks for the controlled self-assembly of nanomaterials. Foldamers are oligomers that exhibit a define tendency to folding to stable 3D structure in solution and possess enormous potential among synthetic self-organizing systems to mimic the regular structural behavior of biomolecules. Virtually unexplored is a subject of self-organization of complex α,β-peptides. That is why, there will be carried out studies in order to increase knowledge regarding design, synthesis and aggregation of peptides containing both α- and β-residues, which can be potentially applied in the synthesis of bionanomaterials with a vast range of applications.
 
The main goal of the project involves rational design of peptide foldamers to be exploited as building blocks for the controlled self-assembly of nanomaterials. Foldamers are oligomers that exhibit a define tendency to folding to stable 3D structure in solution and possess enormous potential among synthetic self-organizing systems to mimic the regular structural behavior of biomolecules. Virtually unexplored is a subject of self-organization of complex α,β-peptides. That is why, there will be carried out studies in order to increase knowledge regarding design, synthesis and aggregation of peptides containing both α- and β-residues, which can be potentially applied in the synthesis of bionanomaterials with a vast range of applications.
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== Conference communications ==
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# 25th Polish Peptide Symposium, Wojanów, 8-12.09.2019 - poster "Enhancing the stability and self-assembly of short coiled-coil peptides by introducing cyclic β-amino acids".
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 +
== Publications ==
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<pubstopic topicid="328">hej</pubstopic>

Latest revision as of 07:57, 23 March 2021

Principal Investigator: Monika Szefczyk

Team:

Ewa Rudzińska-Szostak, PhD, D.Sc.

Daniel Wiczew, MSc, Eng.

Magda Drewniak-Świtalska, Ph.D, Eng.

Scientific Cooperation:

Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology:

Małgorzata Kotulska, PhD, D.Sc, Eng; Marlena Gąsior-Głogowska, PhD, Eng; Natalia Szulc, MSc, Eng.

FTIR, RAMAN

Faculty of Microsystem Electronics and Photonics, Wroclaw University of Science and Technology:

Teodor Gotszalk, PhD, D.Sc, Eng; Krzysztof Gajewski, PhD, Eng; Wojciech Majstrzyk, MSc, Eng. AFM

Faculty of Mechanical Engeineering, Wroclaw University of Science and Technology:

Andrzej Żak, PhD, Eng;

TEM

Division of Biophysics, Faculty of Physics, Institute of Experimental Physics, University of Warsaw,

Agnieszka Bzowska, , PhD, D.Sc; Anna Modrak-Wójcik, PhD

AUC

Faculty of Biochemistry, Molecular Biology and Biotechnology, Wroclaw University of Science and Technology:

Rafał Hołubowicz, PhD, Eng;


Project financed by: National Science Centre, no. 2017/26/D/ST5/00341

Start date: June 2018

Project duration: 36 months

Budget: 630 500,00 zł


Abstract

Bionanomaterials are nowadays one of the fastest growing research area of material engineering. Special attention has been paid to the materials composed of self-organizing structures, such as nucleic acids, hydrocarbons and peptides. Particularly, self-assembling peptides have revealed many advantages over other organic and inorganic aggregates, for instance biological compatibility, rather simple synthesis and low toxicity. Moreover, vast possibility of peptides chemical properties modulation guarantees a range of possible applications. As so, bionanomaterials based on peptide nanostructures have shown to entrap many different bioactive molecules with a controlled release pattern, thus can be applied as drug delivery systems. However, only ability to rational control over the assembly formation, especially influence on type and size of obtained nanostructures, will provide bionanomaterials useful for applications in nanotechnology and synthetic biology.

The main goal of the project involves rational design of peptide foldamers to be exploited as building blocks for the controlled self-assembly of nanomaterials. Foldamers are oligomers that exhibit a define tendency to folding to stable 3D structure in solution and possess enormous potential among synthetic self-organizing systems to mimic the regular structural behavior of biomolecules. Virtually unexplored is a subject of self-organization of complex α,β-peptides. That is why, there will be carried out studies in order to increase knowledge regarding design, synthesis and aggregation of peptides containing both α- and β-residues, which can be potentially applied in the synthesis of bionanomaterials with a vast range of applications.

Conference communications

  1. 25th Polish Peptide Symposium, Wojanów, 8-12.09.2019 - poster "Enhancing the stability and self-assembly of short coiled-coil peptides by introducing cyclic β-amino acids".

Publications

  • Szefczyk, M.; Szulc, N.; Gąsior-Głogowska, M.; Modrak-Wójcik, A.; Bzowska, A.; Majstrzyk, W.; Taube, M.; Kozak, M.; Gotszalk, T.; Rudzińska-Szostak, E.; Berlicki, Ł., Hierarchical approach for the rational construction of helix-containing nanofibrils using α,β-peptides. Nanoscale 2021, 13, 4000. View at Publisher
    Abstract

    The rational design of novel self-assembled nanomaterials based on peptides remains a great challenge in modern chemistry. A hierarchical approach for the construction of nanofibrils based on α,β-peptide foldamers is proposed. The incorporation of a helix-promoting trans-(1S,2S)-2-aminocyclopentanecarboxylic acid residue in the outer positions of the model coiled-coil peptide led to its increased conformational stability, which was established consistently by the results of CD, NMR and FT-IR spectroscopy. The designed oligomerization state in the solution of the studied peptides was confirmed using analytical ultracentrifugation. Moreover, the cyclopentane side chain allowed additional interactions between coiled-coil-like structures to direct the self-assembly process towards the formation of well-defined nanofibrils, as observed using AFM and TEM techniques..